Fig. 6: Nature of IbpA association with mCherry fusion foci by 2D SIM.
From: An experimental framework to assess biomolecular condensates in bacteria
a Fluorescence intensity profile lines of mCherry fusion foci (magenta) and IbpA-msfGFP foci (green). Dashed lines and shading are the mean and standard deviation, respectively, of fluorescence intensities normalized to the mCherry signal. cIagg: n = 348 foci; PopTagSL: n = 540 foci; PopTagLL: n = 547; McdB: n = 295 foci. b 2D SIM images of mCherry fusion proteins foci (magenta) and IbpA foci (green) are shown. Images are representative of three biological replicates. Scale bar: 2 µm. c Fluorescence projection images of mCherry fusion foci and local IbpA-msfGFP signal. Black dashed lines indicate the contour at 0.25, 0.50, and 0.75 of the 2D Gaussian fit amplitude surrounding the mCherry fusion focus. cIagg: n = 84 foci; PopTagSL: n = 506 foci; PopTagLL: n = 457 foci; McdB: n = 217 foci. Source data are provided as a Source Data file.
