Fig. 6: Chemical inhibition of complex I activity in vitro is correlated with lethal activity in the C. elegans RQ-dependent metabolism assay in vivo.
From: Identification of a family of species-selective complex I inhibitors as potential anthelmintics
a Chemical structures of class A (NPD8790), B (STK697993) and C (7732524) benzimidazole analogs; rotenone and fenazaquin, known complex I inhibitors; and fenbendazole, a benzimidazole anthelmintic. b Dose-response curves of each of the six compounds against the in vitro activity of complex I from wild-type C. elegans (N2) mitochondria. c Dose-response curves for each of the six compounds against wild-type L1 C. elegans in the RQ-dependent KCN survival assay. IC50 (b) and LC50 (c) values estimated from dose-response curves in each respective assay are shown for each of the six compounds. All data are the mean of at least three biological replicates; error bars represent SEM. d In vivo LC50 values (c) from the KCN survival assay were plotted against the in vitro IC50 values (b) from complex I enzymatic assay for each compound. A Pearson’s correlation coefficient of 0.94 (strong correlation) was calculated for the relationship between in vitro and in vivo activities.
