Fig. 1: Three fungal species grow optimally at different temperatures, and their transcriptional heat shock responses track their optimal growth temperatures.
From: An adaptive biomolecular condensation response is conserved across environmentally divergent species
a Phylogenetic tree of the Saccharomycetaceae family. The topology was obtained from Kumar et al. 2022. b Growth rate versus temperature, shown as mean +/− standard error, overlaid with a fit of the cardinal temperature model with inflection to experimental data obtained for strains S. cerevisiae BY4742 (purple), S. kudriavzevii FM1389 (green), and K. marxianus DMKU3-1042 (blue). Dotted lines show the temperature of the maximum growth rate for each species. At least two biologically independent cultures were measured for each temperature and species. c Hsp70 fluorescence after 20 minute heat shock at specified temperature with three hours of recovery. Values are plotted as the percent of the maximum response. Each point is the average of at least 5000 cells, controlled for size and normalized to non-heat shocked cells. Dotted lines show the temperature of the maximum measured transcriptional response for each species. d Correlation of the temperatures at which the three species reach their highest maximum specific growth rate from b plotted against the temperature of the maximum Hsp70 response in c. Error bars represent the standard deviation of temperature for the fit of the skew-normal distribution (y-axis) or cardinal temperature model with inflection (x-axis). Solid line shows a linear fit, dashed line shows y = x. Source data are provided as a Source Data file.
