Table 1 Strains and plasmids
From: An adaptive biomolecular condensation response is conserved across environmentally divergent species
Organism/strain | Genotype | Source |
|---|---|---|
S. cerevisiae BY4742 | MATα ura3Δ0 leu2Δ0 his3Δ1 lys2Δ0 | |
S. kudriavzevii FM1389 (original ZP591) | MATα hoΔ::kanMX ura3Δ0 his3Δ0 | |
K. marxianus DMKU3-1042 | MATα | NBRC 104275 (Japan) |
K. marxianus RAK3877 | MATα ura3-1 his5-1 | |
S. cerevisiae yCDK061 | MATα ura3Δ0 leu2Δ0 his3Δ1 lys2Δ0 PAB1-1255-1506::Pab1*1255-1506, MV → A | |
S. cerevisiae yCDK066 | MATα ura3Δ0 leu2Δ0 his3Δ1 lys2Δ0 PAB1-1255-1506::Pab1*1255-1506, MV → I | |
S. kudriavzevii ySMK103 | MATα hoΔ::kanMX ura3Δ0 his3Δ0 SKDZ_05G2450(PAB1)−1255-1506::SKDZ_05G2450(Pab1)*1255-1506, MV → A | This study |
S. kudriavzevii ySMK105 | MATα hoΔ::kanMX ura3Δ0 his3Δ0 SKDZ_05G2450(PAB1)−1255-1506::SKDZ_05G2450(Pab1)*1255-1506, MV → I | This study |
S. kudriavzevii FM1171 | MATa/MATα | |
S. cerevisiae BY4743 | MATa/α ura3Δ0/ura3Δ0 leu2Δ0/leu2Δ0 his3Δ0/his3Δ0 MET15/met15Δ0 LYS2/lys2Δ0 | |
S. cerevisiae yCGT027 | MATα ura3Δ0 leu2Δ0 his3Δ1 lys2Δ0 SSA4::SSA4-mCherry | |
S. kudriavzevii ySMK009 | MATα hoΔ::kanMX ura3Δ0 his3Δ0 SKDZ_05G1830(SSA4)::SKDZ_05G1830(SSA4)-mCherry | This study |
Plasmid | Details | Source |
pSK166 | 8xHis-(TevC)-SKDZ_05G2450(PAB1) in pET28a backbone | This paper |
pSK168 | 8xHis-(TevC)-SKDZ_05G2450(PAB1) MV → A in pET28a background | This paper |
pSK170 | 8xHis-(TevC)-SKDZ_05G2450(PAB1) MV → I in pET28a background | This paper |
pSK172 | pV1382 + SKDZ_05G2450(PAB1) P-domain guide CGGCCGCAGCGGTAGCTTGT | 107 this paper |
pSK173 | pV1382 +SKDZ_05G2450(PAB1) P-domain guide CAACAAATGAACCCAATGGG | 107 this paper |
pSK174 | pV1382 + SKDZ_05G2450(PAB1) P-domain guide TACGGTGTCCCTCCACAAGG | 107 this paper |
pSK180 | 8xHis-(TevC)-KLMA_30299(PAB1) wild type in pET28a background | This paper |
pSK181 | 8xHis-(TevC)-KLMA_30299(PAB1) MV → A in pET28a background | This paper |
pSK182 | 8xHis-(TevC)-KLMA_30299(PAB1) MV → I in pET28a background | This paper |
pSK022 | pCGT07 + SKDZ_05G1830(SSA4)- mCherry repair template carrier | 16 this study |
pSK025 | Cen6-URA + Cas9-PGK1 + SKDZ_05G1830(SSA4) guide CGGAACCGGACCTGCTCCAG | 78 this paper |
pSK026 | Cen6-URA + Cas9-PGK1 + SKDZ_05G1830(SSA4) guide GGTGCTGGAGCAGGCCCAAG | 78 this paper |
pJAR006 | 8xHis-(TevC)-Pab1 in pET28a backbone | |
pJAR033 | 8xHis-(TevC)-Pab1 MV → A in pET28a background | |
pJAR035 | 8xHis-(TevC)-Pab1 MV → I in pET28a background | |
pSK190 | pSSA3-eGFP-CYC1t with Kmarx CEN/ARS, HIS5 | 80 this paper |
