Fig. 6: GLIC-lipids interaction pattern. | Nature Communications

Fig. 6: GLIC-lipids interaction pattern.

From: Cryo-EM structures of prokaryotic ligand-gated ion channel GLIC provide insights into gating in a lipid environment

Fig. 6

Interactions of Lipid 1 with (a) GLICaso-pH7.5 (plum) and (b) GLICpH4.0-C1 (dodger blue), (c) Lipid 2 with GLICaso-pH7.5 are shown. Lipids are depicted in ball-stick representation. d Top view of the TMD (EM density, gray) shows wedging of Lipid 2 (ball-stick) inside the cavity formed by M1, M3, and M4 helices. The wedging of this lipid is observed in acidic pH conditions, and the lipids from GLICpH4.0-O (deep pink) are shown here as a representative. e The interactions of Lipids 3 and 4 with R287 in GLICaso-pH7.5 (plum) are shown. f The interactions of Lipid 5 with Y278 located at M3 helix and with an alternative conformation of R293 on M4 in GLICpH5.5 (green) are shown. The cryo-EM density for R293 is displayed as blue mesh. Interactions of Lipids 2 and 9 in GLICpH4.0-C2 (salmon) (g) and in GLICpH4.0-O (deep pink) (h) are shown. Lipids are shown in ball and stick representations, whereas interacting residues are shown as sticks with corresponding colors. Protein is represented as a cartoon (gray).

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