Table 2 Kinetics parameters of L-alanine uptake induced by Asc1/CD98hc in HeLa cells

From: Structure and mechanisms of transport of human Asc1/CD98hc amino acid transporter

 

L-alanine uptake

 

Km (μM)

Vmax (pmol mg−1 protein min−1)

hAsc1 WT

35 ± 11

4659 ± 370

hAsc1 S56A

88 ± 8 (***)

1105 ± 79 (***)

hAsc1 S246G

48 ± 6 (ns)

1590 ± 152 (***)

hAsc1 Y333F

33 ± 3 (ns)

1328 ± 178 (***)

  1. Data (mean ± s.e.m.) of wild-type and the indicated mutants of Asc1 corresponds to n = 3 independent experiments run in triplicates. A linear model was used to compare conditions after log-transforming the data to obtain p values. P values were adjusted for multiple comparisons using the Benjamini-Hochberg method (see material and methods): ns, non-significant; (***) p < 0.001. WT vs S56A Km, p = 7.20 ∙ 10−5; WT vs S56A Vmax, p = 2.46 ∙ 10−8; WT vs S246G Km, p = 0.104; WT vs S246G Vmax, p = 5.93 ∙ 10−7; WT vs Y333F Km, p = 0.878; WT vs Y333F Vmax, p = 1.06 ∙ 10−7.
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