Fig. 1: Selection of halogenases to acquire a panel of modified precursors. | Nature Communications

Fig. 1: Selection of halogenases to acquire a panel of modified precursors.

From: A modular and synthetic biosynthesis platform for de novo production of diverse halogenated tryptophan-derived molecules

Fig. 1

A Reaction overview for tryptophan halogenases. 5-tryptophan-halogenases: PyrH, XsHal; 6-tryptophan-halogenases: Th-Hal, SttH, Thal; 7-tryptophan-halogenases: RebH, PrnA. B Experimental Design for halogenase panel. Strain sKR-160 containing respective halogenases and flavin reductase Th-Fre were supplied with 1 mM of tryptophan and production formation of respective halogenated tryptophan products (5-, 6-, 7-chloro-/bromo-tryptophan) was observed. C Panel of halogenases with calculated L-tryptophan conversion at varying temperatures: 25 °C, 30 °C, and 37 °C. Data are mean ± S.D.; n = 3 biological replicates. Source Data are provided as a Source Data file.

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