Fig. 7: Modular one-pot de novo co-culture reactions enable halogenated product diversity.

Schematic of the modular one-pot de novo co-culture reactions to enable halogenated product diversity. One strain converts glucose into 5-, 6-, or 7-halogenated tryptophan. The second strain converts the halogenated tryptophan into a halogenated downstream product which is secreted into the media. Boxes with both Br and Cl icons refer to downstream products for which both brominated and chlorinated versions have been detected using each respective downstream enzyme. Boxes without Br and Cl refer to downstream products which were not detected when attempting the specified co-culture.