Fig. 8: De novo production of a wide range of tryptophan-derived halogenated products through modular one-pot co-cultures.

A Production of chloro-specific products, where each heat map square represents the formation (blue) or lack of formation (white) of corresponding halogenated products from biological triplicates picked from individual colonies. New-to-nature molecules are designated with a black star and products produced via de novo microbial synthesis for the first time are designated with a black circle. B production of bromo-specific products, where each heat map square represents the formation (orange) or lack of formation (white) of corresponding halogenated products from biological triplicates picked from individual colonies. New-to-nature molecules are designated with a black star and products produced by microbial synthesis are designated with a black circle. C Confirmation of de novo production of 6-chloro-N-formyl-L-kynurenine (5d), 6-bromo-N-formyl-L-kynurenine (5e), 7-chloro-1-acetyl-3-carboxy-β-carboline (6f), 7-bromo-1-acetyl-3-carboxy-β-carboline (6g), respectively, via LC-MS.