Table 2 List of plasmids used in the study
From: MeCP2 binds to methylated DNA independently of phase separation and heterochromatin organisation
Mammalian expression plasmids | ||
|---|---|---|
Name | Details | Source |
pPYCAG-mCherry-2xCHD | Heterochromatin reporter (dimerised CBX1 chromodomain), N-terminal mCherry tag, Constitutive CAG enhancer/promoter | This study |
pEGFP-Human MeCP2 | Wild-type Human MeCP2, N-terminal EGFP tag, Constitutive CMV enhancer/promoter | |
pEGFP-Mouse MeCP2 | Wild-type Mouse MeCP2, N-terminal EGFP tag, Constitutive CMV enhancer/promoter | |
pEGFP-MeCP2 AT-Hookmut | Mouse MeCP2 AT-Hook mutant (R188G, R190G), N-terminal EGFP tag, Constitutive CMV enhancer/promoter | This study |
pEGFP-MeCP2 ΔMBD | Mouse MeCP2 MBD mutant (Δ99–125), N-terminal EGFP tag, Constitutive CMV enhancer/promoter | This study |
pEGFP-MeCP2 ΔMBD + AT-Hookmut | Mouse MeCP2 MBD and AT-Hook double mutant (Δ99–125, R188G, R190G), N-terminal EGFP tag, Constitutive CMV enhancer/promoter | This study |
pEGFP-MeCP2 Minimal MBD | Mouse Minimal functional MBD (78–162), N-terminal EGFP tag, Constitutive CMV enhancer/promoter | This study |
