Fig. 5: Differences in the holin protein explain reduced host range of PRDcerulean.
From: Diverse and abundant phages exploit conjugative plasmids
a Schematic representation of holin (P35) mutants, and their effect on plaque formation. Gray arrows represent the WT PRD1 holin gene, blue arrows represent the WT PRDcerulean holin gene. Amino acid changes are represented by purple, green, and red marks on the holin gene body. To the right, the corresponding plaque assays of tenfold dilutions on S. entericaP and P. putidaP are shown. Complete replacement of the holin gene, as well as specific mutations in the TMD2&3 restore the plating efficiency of PRDcerulean on S. entericaP. b Diagram of the predicted membrane topology of the holin protein (P35cer). It is predicted to have a short N-terminal periplasmic segment, three transmembrane domains (TMDs), and a longer disordered C-terminal region that extends into the cytoplasm. P = periplasm, IM = inner membrane, and C = cytoplasm. Relevant mutations are colored as in (a).
