Fig. 2: NRP1-deficiency enhances anti-tumor migration and activity of CD8+ T cells. | Nature Communications

Fig. 2: NRP1-deficiency enhances anti-tumor migration and activity of CD8+ T cells.

From: Semaphorin 3A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells

Fig. 2

a Growth curve of B16.F10 cells in Cd4Cre Nrp1+/+, Nrp1Flox/+ and Nrp1Flox/Flox mice (left) and Kaplan-Meier survival curve (right). Dashed lines indicate growth in individual mice, bold line average for group (n = 3–6 mice per group). b Growth curve of LL/2 cells in Cd4Cre Nrp1+/+, Nrp1Flox/+ and Nrp1Flox/Flox mice (left) and Kaplan–Meier survival curve (right). c Growth curve of B16.F10 cells in Cd4Cre Nrp1Flox/+ and Nrp1Flox/Flox mice pre-treated with either anti-CD8 antibody or isotype control. d Enumeration of CD4+ and CD8+ T cells infiltrated into B16.F10 tumors in Cd4Cre Nrp1+/+, Nrp1Flox/+ and Nrp1Flox/Flox mice (n = 6 per group). e Expression levels of activation and exhaustion markers on CD8+ T cells within the tumor 15 days post injection (n = 3–6 in Nrp1Flox/Flox group, 6 in Nrp1Flox/+ group and 4–7 in Nrp1+/+ group). f Experimental setup of mixed bone marrow chimeras in C57BL/6 mice (left) and subsequent enumeration of CD8+ T cells in mice (middle graph). Ratio of CD8+ T cells from Cd4Cre Nrp1Flox/Flox to Nrp1Flox/+ bone marrow derived cells (right graph) (n = 6 mice per group). g Experimental setup using B16.F10 Sema3a KO or Sema3a OE cells (left) and growth curve of cells in untreated mice (right) (n = 8 mice). h Growth curve of B16.F10 Sema3a KO or Sema3a OE cells using similar experimental setup as in (f), but with OT-I treatment at day 7 post-injection (n = 6 mice per group). i Enumeration of OT-I T cells in tumors (left graph) and their ratio of cells, normalized to the number in the B16.F10 Sema3a KO tumor (right) from same experiment as in (g). Abbreviations: ns, not significant. Error bars are means ± SD from 1 (b, d–i), or combined from 2 (c, e) and 4 (a) independent experiments. *P = 0.0312 (f), P = 0.043 (i), **P = 0.0041 (b), P = 0.0091 (c), P = 0.0072 (d), P = 0.008 (h), ****P < 0.0001 (a) by two-way ANOVA (a–d, g, h) or one-way ANOVA (E) or two-tailed paired t-test (f, i). Source data are provided as a Source Data file.

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