Fig. 5: Functional B and T cell phenotype composition using single cell RNA sequencing.
From: Molecular patterns of resistance to immune checkpoint blockade in melanoma
A Uniform Manifold Approximation and Projection (UMAP) plot of 26,053 single cells from one anti-PD1 resistant (PD1res), one anti-CTLA4 resistant (CTLA4res) and two immune checkpoint blockade (ICB) naïve melanomas, with increased B cells. Cell type assignments derived from manual annotation are indicated in the plot. B UMAP of 559 B cells visualizing eight distinct clusters. B cell subsets are indicated in the plot after manual annotation. The scheme of the experimental procedure was created with BioRender.com. C Pie charts describing the fraction of each B cell subset in the four melanomas. D Violin plots of the expression of IGHA1 and IGHG1 in memory-like B cells in the four melanomas. E UMAP of 2921 T cells visualizing 11 clusters, in the four melanomas. T cell subsets are indicated in the plot after manual annotation. F CD8 T cell phenotype fractions based on non-zero expression of PD1 and TCF7 in CD8A or CD8B expressing T cells of all four melanomas. Source data are provided as a Source Data file.
