Fig. 6: T cell phenotypes in immune checkpoint blockade (ICB) resistant melanomas.
From: Molecular patterns of resistance to immune checkpoint blockade in melanoma
A CD3+ and CD8+ T cell fraction using multiplex immunofluorescence shown as boxplots. P-values from Kruskal-Wallis test. B FOXP3+ T cell fraction using multiplex immunofluorescence shown as boxplot. P-value from Wilcoxon test. Test was two-sided. C Multiplex immunofluorescence images from an anti-CTLA4 resistant (CTLA4res) melanoma that has infiltration of FOXP3+ T cells. FOXP3+ T cells are co-localized with CD8+ T cells and marked by an arrowhead. D Multiplex immunofluorescence images from an anti-PD1 resistant melanoma (PD1res) that has a strong infiltration of CD8+ T cells. PD1−/TCF7− double negative CD8+ T cells areas are marked. E Multiplex immunofluorescence images from an ICB naive melanoma that has a strong infiltration of CD8+ T cells. PD1+ CD8+ T cells area is marked. F Boxplots of the fraction of PD1+TCF7- of CD8+ T cells and the fraction of double negative T cells (PD1−/TCF7−) using multiplex immunofluorescence. P-values from Wilcoxon test. All tests were two-sided. Green - ICB naïve (A, B, n = 52; F, n = 50) orange - anti-CTLA4 resistant (CTLA4res) (A, B, F, n = 10), red - anti-PD1 resistant (PD1res) (A, B, F, n = 8), purple - anti-PD1 resistant under BRAFi (PD1res*) (A, B, F, n = 6). Boxplots are displayed with the center-line as median, the box limits as lower and upper quartiles, and with whiskers covering the most extreme values within 1.5 x Interquartile-Range. Representative areas from two tumor cores (1 mm in diameter) per melanoma metastasis were selected in the display items in (C–E). Source data are provided as a Source Data file.
