Fig. 3: No detection of human T or B lymphocytes in the porcine kidney xenografts.

Spatial transcriptomics was performed on serial needle core biopsies of 10-GE porcine kidneys before and after transplantation into a brain-dead human recipient. Biopsies were obtained from either the right (pre-transplant and day 3 samples) or left (day 1 and day 3T) xenografts. Cell type signatures were identified from reference transcriptomes using cell2location (for Pig T cells, top plots in 3a) or expression of individual marker genes (e.g. CD3E and CD19) for adaptive immune cell types that were not well represented in CD45+ immune cells sorted from the xenograft explants (see Fig. 1) and were therefore not included in the reference transcriptomes passed to cell2location. a No detection of human T cell genes in xenograft biopsies at any time point (left) with quantification of gene expression levels (right). Pig T cells were readily detectable in the xenograft (top row). b Calculated total (left) and normalized (right) cell abundance for various pig T cells in the indicated biopsies. Note that cell abundance for human T cells was imputed from expression of hg38-CD3E, ss11-CD19, and hg38-CD19 genes as shown (a). c No detection of human B cell genes in xenograft biopsies at any time point (left) with quantification of gene expression levels (right). Pre-tx pre-transplant. day 3T biopsy was taken on post-transplant day 3 at study termination.