Fig. 5: mGluR regulates the secretion of NPF from EECs.

a Food intake of flies depleted for different glutamate receptors in EECs under 1% L-Glu feeding condition. Red-dash line box indicates the two mGluR^RNAi lines that significantly decreased the food intake. b Under 1% L-Glu feeding condition, representative heatmap records of GCaMP intensity of 10 individual EECs in control and tap^1.3-B-Gal4>mGluR^RNAi flies within 10 min. c Representative images of pANF-EMD staining in EECs of control and tap^1.3-B-Gal4>mGluR^RNAi flies under 1% L-Glu feeding condition. d Representative images of NPF staining in EECs of control and tap^1.3-B-Gal4>mGluR^RNAi flies under 1% L-Glu feeding condition. e–g Quantification of calcium peaks e, pANF-EMD f and NPF g staining in EECs of control and tap^1.3-B-Gal4>mGluR^RNAi flies under 1% L-Glu feeding condition. n = 30 f and =75 g. Data are represented as mean ± SD. Significance was determined using two-sided unpaired t-test (a, e–g). n, number of groups performed for quantification of food intake (5 flies in each group) a, or number of EECs e–g. Source data are provided as a Source Data file. Scale bars, 20 μm.