Fig. 2: Functional validation of WTK7-TM using VIGS and ethyl methane sulfonate (EMS) mutants assay.
a Relative expression levels of WTK7-TM in lines 3D232 and 5BIL-29 treated with BSMV:00 (control), BSMV:WTK7-TMKin I and BSMV:WTK7-TMKin II, respectively, were examined by qRT-PCR. Results represent the means ± SD from three biological replicates (three leaves used per biological replicate) and three technical replicates for each leave (N = 9, unpaired two-tailed t-test). “****” indicates a statistically significant difference (P < 0.0001). b Plants were inoculated with Bgt isolate E09 and representative leaves were photographed at 14 dpi. VIGS1 and VIGS2 showed the two target positions of WTK7-TM in BSMV-VIGS experiments. Three independent experiments were performed. c Line 3D232 and 17 susceptible mutants on WTK7-TM inoculated with Bgt isolate E09 and representative leaves were photographed at 14 dpi. Three independent experiments were performed. d Susceptible EMS mutants carrying single nonsense or missense mutations in the WTK7-TM gene. Black straight lines indicate introns, and rectangles indicate coding exons. Domains Kin I and Kin II, and the transmembrane region of WTK7-TM are highlighted in orange, yellow, and cyan rectangles, respectively. Short vertical lines indicate the mutated positions. The DNA sequence (D.) changes, and their predicted effects on the translated protein (P.) are indicated below the mutants. Mutation names in red, purple, and black indicate nonsense, splice site, and missense mutations, respectively. P-values and source data are provided as a Source Data file.
