Fig. 3: Transgenic validation of WTK7-TM. | Nature Communications

Fig. 3: Transgenic validation of WTK7-TM.

From: A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew

Fig. 3

a Structure of ProWTK7-TM:WTK7-TM used for transgenic assay. The ProWTK7-TM:WTK7-TM construct contains the 3277 bp full-length WTK7-TM coding region, as well as 3311 bp upstream of the start codon and 4153 bp downstream of the stop codon. LB, left border; RB, right border. b The resistance performance of ProWTK7-TM:WTK7-TM–transformed transgenic plants. Transgenic T1 wheat (about 15 plants per family) was challenged with Bgt isolate E09 at the two-leaf stage. Representative leaves were photographed at 12 dpi. PCR results as positive (+) or negative (-) for DNA amplification (upper) and RNA expression (lower) of the WTK7-TM gene. Three independent experiments were performed. c The relative expression levels of WTK7-TM in transgenic wheat. Leaves of seedling plants at the two-leaf stage were collected. TaActin was used as an endogenous control. Expression values are means ± SD from three biological replicates (three leaves used per biological replicate) three biological replicates (three leaves used per biological replicate) and three technical replicates for each leave (N = 9). There was no expression in non-transgenic plants. Error bars are standard errors of the mean. Source data are provided as a Source Data file.

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