Fig. 3: De novo biosynthesis of the bergenin precursor 4-OMGA-Glc from glucose in E. coli.
From: Unravelling and reconstructing the biosynthetic pathway of bergenin
a The construction of the GA-producing module and OMT-CGT module using different plasmids. b Evaluation of the engineered strains A1–A6 for the titers of 4-OMGA-Glc (4) and metabolic intermediates in shake flask fermentation for 48 h. UbiC, chorismate lyase; PobA**, p-hydroxybenzoate hydroxylase with Y385F and T294A mutation; pT7, T7 promoter; RBS, ribosome binding site; T, terminator. All data represent the means of three parallel experiments and error bars show standard deviation. Statistical analysis was performed by using the Student′s t test. P value for each comparison from left to right then top to bottom in b): 0.0408 (*), 0.0475 (*), 0.0106 (*), 0.0047 (**), 0.0081 (**), 0.0048 (**), 0.0001 (**). Source data are provided as a Source data file.
