Fig. 6: Validation of the clinical feasibility of SCOPE.

a Workflow of qPCR assay and our CRISPR-based assay for testing of clinical samples. b The corresponding fluorescent intensities at 10 min for 102 clinical samples tested by SCOPE. c The receiver operating characteristic curve for 102 clinical samples tested by SCOPE. d Clinical samples RT-qPCR Ct value plotted against fluorescent intensity readout by CPod. e Correspondence of qPCR and SCOPE results for the monkeypox virus positive samples, herpes simplex virus (HSV) samples, and negative samples.