Fig. 1: Oxidative stress induces LBP upregulation and LD translocation.

a A schematic illustrating the research strategy adopted in this study. The image was created using materials supplied by Ge Pan from the Central Academy of Fine Arts, China, and illustrated by Qilun Zhang. The use of these materials has been authorized. b A multivariate bubble plot comparing changes in LD-localized protein and RNA expression levels after a 24-h fast. Two-tailed student’s t-test (each group n = 3, biologically independent). c Western blot analysis of LBP in liver tissue or liver LDs from 8-week-old mice receiving no treatment (NT) or a 24-h fast. d Changes in LBP mRNA expression after treatment with 1 μM Bodipy C12, 2 μM PC, or 60 μM PA for 24 h. Shown are means ± s.d., one-way ANOVA (n = 3, biologically independent). e Changes in LBP mRNA expression after treatment of HepG2 cells with 4 μg/ml tunicamycin for 8 h, 1 mM hydrogen peroxide for 2 or 4 h, nutritional-starvation for 3 h, or 50 °C heat shock for 1 h. Shown are means±s.d., one-way ANOVA (n = 3, biologically independent). f Confocal microscope co-staining images of LBP (green), PLIN2 (violet), and DAPI (blue) captured 16 h after the treatment with Bodipy C12 (orange), 1 μg/ml LPS for 8 h, and the other treatments as described in (e). Scale bar = 5 μm. Experiments repeated three times independently with similar results.