Fig. 4: The antioxidant activity of LBP.
a A nine-quadrant plot depicting the combined analysis of the liver transcriptome and proteome of WT and LBPKI/KI mice fed with HFD for 16 weeks. Each group n = 3, biologically independent. b Go term enrichment analysis of the differential proteins in the third quadrant (upregulated) of (a), using different colors to represent different functions: yellow-lipid transport, red-antioxidant, and purple-ER localization. Bar graph shows the -log10 of P-value (hypergeometric test). c Go term enrichment analysis of the differential proteins in the seventh quadrant (downregulated) of (a), using green color to represent lipid metabolism. Other colors represent the same meanings as in (b). Bar graph shows the -log10 of P-value (hypergeometric test). d Bar graph shows the relative liver protein carbonylation levels of 16-week HFD WT, LBPKI/KI, and LBP−/− mice. Shown are means ± s.d., one-way ANOVA, (n = 3, biologically independent). e Bar graph shows the relative liver protein 3-nitrotyrosine (3NT) levels of 16-week HFD WT, LBPKI/KI, and LBP−/− mice. Shown are means ± s.d., one-way ANOVA, (n = 3, biologically independent). f Bar graph shows the relative liver malondialdehyde (MDA) levels of 16-week HFD WT, LBPKI/KI, and LBP−/− mice. Shown are means ± s.d., one-way ANOVA, (n = 3, biologically independent). g The bar graph depicts the level of DCFH-DA detected in HepG2 cells following 3 h of nutrient starvation after overexpression of mCherry or LBP-mCherry. Shown are means ± s.d., two-tailed unpaired t-test, (n = 3, biologically independent). h Confocal data showing the co-localization of non-oxidized (violet), oxidized (red) LDs, Bodipy C12 (orange), and GFP/LBP-GFP (green) in 3-h-starved HepG2 cells. Scale bar = 5 µm. Experiments repeated three times independently with similar results.
