Fig. 7: LBP upregulation drives obesity and is rescued by antioxidant treatment. | Nature Communications

Fig. 7: LBP upregulation drives obesity and is rescued by antioxidant treatment.

From: Lipopolysaccharide binding protein resists hepatic oxidative stress by regulating lipid droplet homeostasis

Fig. 7

a Serum TG and FFA levels in 8-week-old mice before and after Poloxamer 407 injection. Means ± s.d., 2way ANOVA (n = 3, biologically independent). b Morphological comparison of LBPKI/KI, LBP−/− and WT mice fed on a 16-week high-fat diet. c Fasting blood glucose levels of mice in (b). Means ± s.d., two-tailed unpaired t-test (n = 6, biologically independent). d Fasting insulin levels of mice in (b). Means ± s.d., two-tailed unpaired t-test (n = 3, biologically independent). e Serum glucose levels during insulin tolerance tests in mice on a 22-week high-fat diet. Means ± s.d. (n = 5, biologically independent). f Area under curve (AUC) analysis of (e). Means ± s.d., two-tailed unpaired t-test (n = 5, biologically independent). g Serum glucose levels during glucose tolerance tests in mice on a 22-week high-fat diet. Means ± s.d. (n = 4 biologically independent). h AUC analysis of (g). Means ± s.d., two-tailed unpaired t-test (n = 4, biologically independent). i PAS staining of mice liver sections, scale bar = 20 μm. Three independent experiments with similar results. j Mitochondrial respiration rates in control/LBP-overexpressing cells with BPTES. Means ± s.d. (n = 3, biologically independent). k Statistical analysis of (j). Means ± s.d., 2way ANOVA (n = 3, biologically independent). l Mitochondrial respiration rates in control/LBP-overexpressing cells with UK5099. Shown are means ± s.d. (n = 3, biologically independent). m Statistical analysis of (l). Means ± s.d., 2way ANOVA (n = 3, biologically independent). n Mitochondrial respiration rates in control/LBP-overexpressing cells with Etomoxir. Shown are means ± s.d. (n = 3, biologically independent). o Statistical analysis of (n). Means ± s.d., 2way ANOVA (n = 3, biologically independent). p Respiratory exchange ratio of mice in a metabolic cage.Means ± s.d. (n = 5, biologically independent). q Body weight curves of mice with/without NAC treatment. Means ± s.d., 2way ANOVA (n = 5–6, biologically independent). r Body fat ratio of mice with/without NAC treatment. Means ± s.d., 2way ANOVA (n = 5, biologically independent). s Body fat ratio of mice with/without 24 h fasting. Means ± s.d., 2way ANOVA (n = 6, biologically independent). t Serum TG levels in 24 h fasted LBPKI/KI mice with/without NAC recovery. Means ± s.d., two-tailed unpaired t-test (n = 3–4, biologically independent). u FFA levels in 24 h fasted LBPKI/KI mice with/without NAC recovery. Means ± s.d., two-tailed unpaired t-test (n = 3–4, biologically independent). v Body weight curves of mice under a ketogenic diet. Means ± s.d., 2way ANOVA (n = 6, biologically independent).

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