Fig. 7: Colon-specific reprogramming of donor Treg after BMT.

a UMAP embedding of only colon-derived donor Treg cells (from n = 3 animals receiving the same graft) analyzed by scRNA-seq and colored by unsupervised clustering (Louvain). b Violin plots showing the expression distributions of marker genes across colon Treg clusters. c Expression of gene signatures (upper panels; genes listed in Supplementary Table 1) or selected marker genes (lower panels) across the UMAP embedding. Expression levels are in log₂ scale and color coded as shown in the corresponding legends. d RNA velocity estimation is visualized on the pre-defined UMAP embedding. (e) Ratios of colon-enriched Trbv segments 12-1, 12-2, 26 compared to all other Trbv segments across sub-clusters in the UMAP embedding (left panel) or their enrichment across sub-clusters are shown (right panel; two-sided Fisher’s exact test with Benjamini–Hochberg correction). f Volcano plot highlighting genes differentially expressed between Treg expressing one of the three enriched Trbv segments (Trbv12-1, 12-2, 26) or any other Trbv segment (Wilcoxon Rank Sum test (two-sided), Bonferroni correction). Hash-dotted horizontal lines mark an FDR value of 0.05 (y-axis) and the log2FC thresholds of ±0.25 (x-axis). Selected genes are marked by coloring (red:up-/blue:down-regulated), triangles: extremely low p values were shifted to the indicated value. e, f Source data are provided as a Source Data file.