Fig. 1: HSF5 is expressed during meiotic prophase in the mouse testis. | Nature Communications

Fig. 1: HSF5 is expressed during meiotic prophase in the mouse testis.

From: Atypical heat shock transcription factor HSF5 is critical for male meiotic prophase under non-stress conditions

Fig. 1

a Genomic view of MEIOSIN and STRA8 binding peaks over the Hsf5 locus. Genomic coordinates derived from NCBI. To specify testis specific transcription, RNA polymerase II ChIP-seq in the testis is shown5. b Schematic diagram of domain structure in six members of mouse heat shock factor (HSF) protein family. Domain name and the number of the amino acid residues are assigned according to Uniprot. DBD DNA-binding domain, HR heptad repeat, IDR Intrinsically disordered region, DD D domain, RD Regulatory domain, 9aaTAD; Transactivation motif. c SEC-MALS profiles of MBP-full length HSF5 (left) and HSF5-DNA binding domain (a.a. 1-209) (right) at room temperature (blue) and after heat treatment at 42 °C for 30 min (red). Thin and bold lines show the refractive index (RI) profile and mass plot, respectively. d The tissue-specific expression pattern of Hsf5 was examined by RT-PCR. Testis RNA was obtained from 3 weeks old (3w) and 8 weeks old (8w) male mice. Ovary RNA was obtained from adult 8 weeks old (8w) female mice. RT- indicates control PCR without reverse transcription. The data was acquired from two separate experiments. e The expression patterns of Hsf5 in the embryonic ovary (E12.5-E18.5, n = 1 for each) and testis (3w, n = 1) were examined by RT-qPCR. Average values normalized to E12.5 ovary are shown with SD from technical triplicates.

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