Fig. 1: Cloning and functional characterization of Rpp6907-7.
From: A pair of atypical NLR-encoding genes confers Asian soybean rust resistance in soybean
a Genetic and physical maps of Rpp6907. (I) Graphical representation of the SX6907 annotated sequences. (II) Comparison of orthologous regions in the reference genome Wm82.a2. v1. b Pathogen infection assay using SX6907, T1 homozygous Tianlong1, T1 Hemizygous, T1 null, T1 Rpp6907-7-RNAi transgenic SX6907 plant, RNAi-GFP, and Tianlong 1. The representative images from a single replicate of ten independent experiments are shown. c Rpp6907-7 T2 events confer complete spectrum resistance to all ASR populations (U.S. and Brazil strains) tested under greenhouse conditions. The representative images from six independent experiments are shown. d, e The Rpp6907-7 events showed no obvious negative phenotypes in two genetic backgrounds. The representative images from a single replicate of three independent experiments are shown. f Rpp6907-7 T2 events showed no obvious negative phenotypes in field conditions. One plot with two rows per expression cassette and null or wild type were photographed at 5 m above the plant canopy in the R2-R3 stages. Each genetic background was conducted with four replicates. g Rpp6907-7 T2 (E1–E3) events showed no significant difference in yield (in grams) compared to null and wild type in field conditions in complete random design. Data are shown as means ± SD (n  =  10 replicates in WT, n = 3 replicates in null, E1–E3). Statistical analysis was performed using a two-tailed Student’s t test. Source data are provided as a Source Data file.
