Fig. 3: Molecular characteristics, tissue distribution, and protein structure of Rpp6907-7.

a Structure of Rpp6907-7 used for the complementation assay. b Expression of Rpp6907-7 in different tissues of SX6907 plants. Values are the means ± SD (n = 3 repeats with five individuals per sample). c Expression of Rpp6907-7 in SX6907 after inoculation with SS4. Values are the mean ± SD (n = 3 repeats); *P < 0.05; (two-tailed Student’s t test). d Subcellular localization of Rpp6907-7. Co-localization of Rpp6907-7:: GFP with the mCherry marker. Bar = 20 µm. e The Rpp6907-7 and fusion proteins were transiently expressed in N. benthamiana. The numbers in parentheses indicate the numbers of leaves displaying cell death out of the total number of leaves infiltrated. Western blots probed with anti-GFP antibodies show the typical expression of all tested proteins. Expected protein size was 30 kDa for EV-GFP, 130 kDa for Rpp6907-7:GFP and Rpp6907-784:GFP. Modified ponceau staining is shown to indicate loading. f Y2H assay assessing the interaction of Rpp6907-7 CC domain. The constructs were co-transformed into yeast cells and grown on selective dropout medium as indicated. Western blots probed with anti-HA and anti-cMyc antibodies show protein accumulation of all tested proteins in Y2H experiments. Unrelated protein SS12 (Glyma.14G199400) as negative controls to test the specificity of the interactions detected. Expected protein size was 18.6 kDa for AD, 40 kDa for CC-AD, 66 kDa for LRR-AD, 50 kDa for NB-ARC-AD, 43 kDa for CC-BD and S12-BD. Modified ponceau staining is shown to indicate loading. 3-amino-1,2,4-triazole (3-AT); DO Supplement -His/-Leu (DDO); DO Supplement -His/-Leu/-Met (TDO). g BiFC assay shows the interaction of Rpp6907-7 CC domain in N. benthamiana. Unrelated protein SS12 as negative controls to test the specificity of the interactions detected. Western blots probed with anti-HA and anti-Flag antibodies show protein accumulation of all tested proteins in BiFC experiments. Expected protein size was 33 kDa for Rpp6907-7CC-YFPC, 12 kDa for EV-YFPC, 39 kDa for Rpp6907-7CC-YFPN and SS12-YFPN. Modified ponceau staining is shown to indicate loading. All images are representative of results obtained from three independent experiments with similar results. Source data are provided as a Source Data file.