Fig. 4: Rpp6907-4 heterodimerizes interact with Rpp6907-7 and suppresses Rpp6907-7-mediated resistance.

a Rpp6907-4 suppresses HR induced by Rpp6907-7 in N. benthamiana leaves. The utilization of fusion GFP and mCherry were employed for the purpose of detecting the typical expression of the target protein. The numbers in paren theses indicate the numbers of leaves displaying cell death out of the total number of leaves infiltrated. Western blots probed with anti-HA and anti-mCherry show protein accumulation of all tested proteins. Expected protein size was 130 kDa for Rpp6907-7:GFP, 74 kDa for Rpp6907-4:mCherry. Modified ponceau staining is shown to indicate loading. b Structure of Rpp6907-4. c Relative expression of Rpp6907-7/Rpp6907-4 in SX6907 after inoculation and non-inoculation with SS4. Values are the mean ± SD (n = 3 repeats); *P < 0.05 (two-tailed Student’s t test). d Subcellular localization of the fused Rpp6907-4: GFP in N. benthamiana leaf cells. Bar = 20 µm. e Localization patterns of Rpp6907-7 and Rpp6907-4. N. benthamiana leaves were transformed with combinations of constructs for the expression of Rpp6907-7: GFP, Rpp6907-4: mcherry. Bar = 20 µm. f The CC domain of Rpp6907-7 and the LRR domain of Rpp6907-4 interact in yeast. The constructs were co-transformed into yeast cells and grown on selective dropout medium as indicated. Western blots probed with anti-HA and anti-cMyc show protein accumulation of all tested proteins. Expected protein size was 18.6 kDa for AD, 31 kDa for Rpp6907-4-CC-AD, 28 kDa for Rpp6907-4-LRR-AD, 48 kDa for Rpp6907-4-NB-ARC-AD, 43 kDa for Rpp6907-7 CC-BD, 69 kDa for S12-LRR-BD. Modified ponceau staining is shown to indicate loading. g The Rpp6907-7 CC domain and Rpp6907-4 LRR domain hetero-complexes were visualized by BiFC. Unrelated protein SS12 as negative controls to test the specificity of the interactions detected. Western blots probed with anti-HA and anti-Flag show protein accumulation of all tested proteins. Expected protein size was 39 kDa for Rpp6907-7CC-YFPN, 22 kDa for Rpp6907-4-LRR-YFPC, 39 kDa for SS12-YFPC. Modified ponceau staining is shown to indicate loading. All images are representative of results obtained from three independent experiments with similar results. Source data are provided as a Source Data file.