Fig. 3: NGFR is positively associated with osteochondral differentiation of human skeletal progenitor cells.

a The UMAP plot of 5 weeks post conception (Carnegie stage 13) human limb bud and 8 WPC (CS22) human long bone. b The dot plot showing expressions of representative genes in each cluster. Clusters 0–4 can be grouped as skeletal lineage cells based on their expression of PRRX1 and PDGFRA. c The circle plot showing the aggregated cell–cell communication network and the total interaction strength (weights) between any two skeletal cell clusters. d Violin plots showing the cumulative expression of NGFR and PRRX1 in skeletal lineage cells of CS13 limb bud and CS22 long bone. e Characterization of the expression of NGFR, SOX9, RUNX2, and ACAN in each cluster of skeletal lineage cells, split by the groups of CS13 limb bud and CS22 long bone.