Fig. 5: Loss of NGFR impairs bone formation in the context of inflammation. | Nature Communications

Fig. 5: Loss of NGFR impairs bone formation in the context of inflammation.

From: Nerve growth factor receptor limits inflammation to promote remodeling and repair of osteoarthritic joints

Fig. 5

a NGFR ablation exacerbated the decrease of alkaline phosphatase activity induced by inflammatory cytokines. Vec, empty vector; sg2, CRISPR vector expressing single guide RNA 2 to mediate Ngfr deletion. n = 3. b, c NGFR perturbation altered SMAD1 and RANKL in TNF-α-treated cells. CRISPRa, CRISPR-mediated activation of Ngfr. n = 3 independent experiments. d–f NGFR deletion downregulated the marker gene expression in BMP-SMAD1 signaling. Data are presented as mean values ± SD. n = 2 independent experiments. Two-way ANOVA. g–i OA-induced upregulations of SMAD1 and p-SMAD1 were significantly attenuated by NGFR deficiency in OA joints. Data are presented as mean values ± SD. n = 5 independent experiments. Two-way ANOVA. j, k NGFR deletion downregulated RUNX2 protein level in OA joints. Scale bar: 100 μm. Data are presented as mean values ± SD. n = 5 independent experiments. Unpaired two-sided Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.

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