Fig. 8: NGF-NGFR restricts the TNF/NF-κB signaling. | Nature Communications

Fig. 8: NGF-NGFR restricts the TNF/NF-κB signaling.

From: Nerve growth factor receptor limits inflammation to promote remodeling and repair of osteoarthritic joints

Fig. 8

a NGFR deletion enhanced TNF-induced phosphorylation of p65. v, empty vector; 2–8, different sgRNAs targeting Ngfr. n = 3 independent experiments. b NGF reduced TNF-induced p-p65, which required NGFR. v vehicle. T, TNF. N, NGF. T + N, TNF + NGF. Cells were treated with indicated growth factor/cytokine for 15 min. sgRNA #2 was used to generate NGFR KO. n = 3 independent experiments. c NGFR overexpression reduced p-p65 and p-IκBα in C2C12 cells treated with TNF for 24 h. R, RFP. N, hNGFR-RFP. n = 3 independent experiments. d NGFR overexpression decreased p-p65 and p-IκBα induced by TNF treatment in non-skeletal HEK293 cells. n = 3 independent experiments. e NGF treatment decreased NF-κB activation induced by TNF in HEK293 cells. n = 3 independent experiments. Source data are provided as a Source Data file.

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