Fig. 2: CXCR2 is expressed in mouse sensory neurons innervating the ankle joint. | Nature Communications

Fig. 2: CXCR2 is expressed in mouse sensory neurons innervating the ankle joint.

From: CXCL5 activates CXCR2 in nociceptive sensory neurons to drive joint pain and inflammation in experimental gouty arthritis

Fig. 2

A PCR showing the expression of Cxcr1 and Cxcr2 genes in mouse DRG (left panel) and spleen (right panel). B Immunostaining showing CXCR2 expression in ankle joint-innervating DRG neurons labeled by retrograde tracer WGA. Scale bar = 100 µm. C Pie graphs showing the % of NeuN+ cells showing WGA+ (left), % of NeuN+ cells showing CXCR2+ (middle), and % of WGA+ cells showing CXCR2+ (right). The numbers in parenthesis indicate the number of cells included. D Immunostaining showing the absence of CXCR2 staining in DRG neurons from global Cxcr2 gene-deficient (Cxcr2−/−) mice. Scale bar = 100 µm. E Immunostaining of CXCR2 with markers for satellite glial cells (GFAP) in mouse DRG. Right panel denotes quantification. Scale bar = 50 µm. F Immunostaining of CXCR2 with markers for non-peptidergic (IB4), peptidergic (CGRP), and large-diameter (NF200) sensory neurons in mouse DRG. Scale bar = 100 µm. Pie graph quantification is shown on the right. Three sections were obtained from each mouse, calculated, and averaged. Data were pooled from four mice/group.

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