Fig. 2: General properties of recombinant β-NGAs.

a SDS-PAGE analysis of recombinant β-NGAs, stained with Coomassie brilliant blue. Effect of (b) pH and (c) metal ions on the enzymatic activity of recombinant β-NGAs. d Effect of temperature on enzymatic activity of recombinant β-NGAs. e Protein thermal shift data of recombinant β-NGAs. f s-v plots of recombinant β-NGAs. All values represent the mean of triplicate measurements.