Fig. 3: Hydrolysis of oligosaccharides by recombinant β-NGAs. | Nature Communications

Fig. 3: Hydrolysis of oligosaccharides by recombinant β-NGAs.

From: Genetic and functional diversity of β-N-acetylgalactosamine-targeting glycosidases expanded by deep-sea metagenome analysis

Fig. 3

a Thin layer chromatography analysis of the standard oligosaccharides and sugars. Lanes 1. GM1a ganglioside sugar; 2. GA1 ganglioside sugar; 3. GM2 ganglioside sugar; 4. GA2 ganglioside sugar; 5. Gb5 globopentaose; 6. Gb4 tetraose; 7. Globo-H hexaose; 8. Forssman antigen pentaose; 9. SSEA-4 hexaose; 10. GM1b ganglioside sugar; 11. 3’-Siaryl-N-acetyllactosamine; 12. 3’-Siaryllactose; 13. Globotriose; 14. Galacto-N-biose; 15. Lactose; 16. Galactose; 17. GalNAc. The structures and the Rf values of the standard oligosaccharides and sugar were listed in the Supplementary Table 1. b–k Thin layer chromatography demonstrates the hydrolysis of oligosaccharides by NgaCa (b), NgaMg (c), NgaAt (d), NgaDssm (e), NgaBa (f, g), NgaBf (h), NgaBl (i), NgaLy (j), NgaP2 (k). The oligosaccharides in each lane were arranged in the same order as in the standard TLC.

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