Fig. 1: Secretome analysis of pancreatic CSCs by quantitative SILAC/mass-spectrometry and transcriptomic analysis identifies WNT signalling pathway to be elevated in CSCs. | Nature Communications

Fig. 1: Secretome analysis of pancreatic CSCs by quantitative SILAC/mass-spectrometry and transcriptomic analysis identifies WNT signalling pathway to be elevated in CSCs.

From: The pRb/RBL2-E2F1/4-GCN5 axis regulates cancer stem cell formation and G0 phase entry/exit by paracrine mechanisms

Fig. 1

a Schematic depiction of the SILAC/mass-spectrometry experimental outline. b Principal Component Analysis (PCA) of secretome samples shows differences between the secretomes of cell types. c Pathway enrichment analysis indicates elevated WNT signalling in CSCs. d WNT ligand secretion is increased in CSCs compared to non-CSCs. e Promoter luciferase analysis with a construct containing TCF/LEF sites upstream of a luciferase reporter (M50 Super 8x TOPFlash) indicates elevated β-catenin transcriptional activity in pancreatic CSCs compared to non-CSCs and hESCs. Data are presented as mean values ± SD. N = 3 independent experiments. Statistical analysis was performed by 2-way ANOVA with multiple comparisons with Tukey correction. f Hierarchical clustering analysis of correlation coefficients for gene expression in RNA-seq samples. g Volcano blot of differential gene expression shows elevated expression of WNT ligands in pancreatic CSCs compared to non-CSCs. h Marker expression in different cell types in PDAC patient tumour sample RNA-sequencing data. i Schematics showing higher expression of WNT ligands in pancreatic CSCs compared to non-CSCs. Source data are provided as a Source Data file.

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