Fig. 2: The impacts of salt stress on bacterial diversity and composition of wild soybean based on 16S rRNA gene amplicon data.

a The impacts of salt stress on bacterial diversity (Shannon index) of bulk soil, rhizosphere soil and root after 1, 7 and 14 days of salt stress (n = 4 biologically independent samples, mean ± SEM). Different letters above the boxes of each compartment indicate a significant difference at P < 0.05 (one-way ANOVA with correction by Tukey’s HSD test, P-values are shown in source data). Abbreviation: Rh, rhizosphere. b PCoA ordination of the Bray-Curtis dissimilarity matrix (OTU level) for control and salt treatments across bulk soil, rhizosphere soil and root after 1, 7 and 14 days of salt stress. Statistical analysis was performed using ANOSIM (analysis of similarities). c Percent relative abundance of the top 15 most abundant orders for bulk soil, rhizosphere soil, and root across control and different levels of salt stress. Different significance levels between control and each salt treatment are marked with asterisks (*P < 0.05, **P < 0.01 and ***P < 0.001, two-sided Student’s t-test, P-values are shown in source data). Low abundance orders (< 1.5%) with significant difference are not marked with asterisks. White asterisks indicate the relative abundance of this order is lower in salt treatments than that in control group. Dark asterisks indicate the relative abundance of this order is higher in salt treatments than that in control group. Source data are provided as a Source Data file.