Fig. 1: MYADM is an essential host factor for human parechoviruses.
From: MYADM binds human parechovirus 1 and is essential for viral entry
a CRISPR screens for human parechovirus A1 (PeV-A1) and A2 (PeV-A2) in heparan-sulfate/sialic acid deficient HT-29 cells (HT29-DKO, ∆EXTL3 and ∆SLC35A1). Left: Bubble plot illustrating enriched genes based on log2(fold change) (LFC) in the PeV-A1 or PeV-A2-selected population, with genes having FDR < 0.01 depicted in dark gray. Right: Distribution of LFC of sgRNAs from the entire library (top), and sgRNAs enrichment targeting indicated genes overlaid on gray lines representing the distribution of all sgRNAs (bottom). b Time course of nLuc-expressing PeV-A1 infection in HT29-DKO and HuTu80 wild type (WT), MYADM knockout (∆MYADM), and HA-tagged MYADM-complemented MYADM knockout (∆MYADM + MYADM-HA) cells at MOI 0.1. Data are presented as mean values +/− SD, n = 3 biologically independent samples. c RT-qPCR quantification of PeV-A1 and PeV-A3 RNA in infected HuTu80 and 293FT WT, ∆MYADM, and ∆MYADM + MYADM-HA cells at MOI 0.1 at 8 hpi or 48 hpi, respectively. Data are presented as mean values +/− SEM, n = 3 biologically independent samples, multiple two-sided unpaired t tests with the Holm–Šídák’s method for multiple comparison, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. d RT-qPCR quantification of PeV-A1 and PeV-A3 RNA in infected A549 WT, ∆MYADM, and ITGB6 knockout (∆ITGB6) cells at MOI 0.1 at 48 hpi. Data are presented as mean values +/− SEM, n = 3 biologically independent samples, two-way ANOVA with Šídák’s multiple comparison test, ****P < 0.0001; ns not significant. Source data are provided as a Source Data file.
