Fig. 2: MYADM is required for infection of primary intestinal organoids.
From: MYADM binds human parechovirus 1 and is essential for viral entry
a Wild type (WT) and two MYADM knockout human colonoid lines (∆MYADM #11 and ∆MYADM #22) were infected with PeV-A1 or K26GFP (HSV-GFP) and stained for double-stranded RNA or GFP, respectively. Uninfected colonoids (Mock) served as negative controls. Right panels illustrating increased magnification of square boxes in the left panel. b Quantification of PeV-A1 infection in colonoids using RT-qPCR and plaque assay over time. Data are presented as mean values +/− SEM, n = 3 or 4 biologically independent samples. c Quantification of K26GFP (HSV-GFP) infection in colonoids using RT-qPCR and plaque assay over time. Data are presented as mean values +/− SEM, n = 3 biologically independent samples. Source data are provided as a Source Data file.
