Fig. 3: Template matching locates the 80S ribosome with high spatial and rotational accuracy.

a Tomogram slice showing abundant ribosomes. b Slice of the z-score map obtained from template matching using the template of the 80S ribosome shown in (g). c Superimposition of the peaks obtained from template matching (blue circles; sampled every 5 degrees and with a cross-correlation threshold z-score\(\ge\)30) and the high-confidence localizations obtained from an expert multiple-step alignment using Relion⁴⁰ reported in ref. ⁴. d Percentage of the high-confidence Relion particles detected within 10 nm of the TM peaks as a function of the rotational sampling (i.e., number of orientations). e, f Histograms of angular (e) and Euclidean distances (f) from the TM peaks to the annotated Relion particles, respectively, each obtained for a 5-degree angular sampling. g Template for 80 S ribosome. h, i Ribosome structure obtained by averaging the particles from TM from (c) (h, no further processing) superimposed on template (i). Source data are provided as a Source Data file.