Fig. 4: Template matching for the segmentation of membranes in 3D.

Results in the top, middle, and bottom row were obtained for templates constructed from a simulated atomic membrane and STAs of the nuclear membrane with diameters of 50 (small STA) and 100 (large STA) voxels, respectively (8.704 Å/voxel). The results from left to right correspond to increasing angular sampling of 20, 10, and 2 deg, respectively. Note that the peaks at the upper right corner originate from a highly curved vesicle while the two stripes on the right-hand side of the cross-correlation maps (z-scores) are a microtubule and not a membrane (see Fig. 1). Source data are provided as a Source Data file.