Fig. 2: Macrophage (Mφ)-specific deficiency of ATG7 aggravates the inflammatory response in AKI mice. | Nature Communications

Fig. 2: Macrophage (Mφ)-specific deficiency of ATG7 aggravates the inflammatory response in AKI mice.

From: Autophagy-deficient macrophages exacerbate cisplatin-induced mitochondrial dysfunction and kidney injury via miR-195a-5p-SIRT3 axis

Fig. 2

a Representative images and quantification of F4/80 (red) in kidney sections for Mφ detection. Scale bar, 20 µm, n = 6 biological replicates for each group. b, c The phenotype of Mφ in kidney tissues was characterized by flow cytometry (b) (M1: CD11c + CD206-; M2: CD206 + CD11c-) and RT‒PCR (c) (M1: CD86, iNOS, FasL); M2: CD163 and Arg1). n = 6 biological replicates for each group. d Immunohistochemical staining for TNF-α in paraffin-embedded kidney sections. Scale bar, 50 µm, n = 6 biological replicates for each group. The mRNA levels (e) and protein levels (f) of inflammation-related genes were measured. ACTIN was used as the loading control. The data are the means ± SEMs. All statistical analysis were performed by two-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source data file.

Back to article page