Fig. 4: The GR/LSD1 complex is required to trigger the expression of genes involved in starvation-induced proteolysis upon food deprivation.
From: LSD1 inhibition circumvents glucocorticoid-induced muscle wasting of male mice
a Tag density map of LSD1, GR, H3K9me1 and H3K9me2 in skeletal muscles of mice fed or fasted for 24 h, +/− 5 kb from the LSD1 peak center and corresponding average tag density profiles. b Localization of LSD1, GR, H3K9me1 and H3K9me2 in skeletal muscles of mice fed or fasted for 24 h at indicated loci. c, d Representative western blot (c) and corresponding quantification (d) of LSD1 and GR protein levels in gastrocnemius muscle extracts from 12-week-old wild type mice fed or food deprived for 12, 24 or 48 h. GAPDH was used as a loading control. N = 2 representative mice (c) out of 7 mice (d). Mean ± SEM. One-way ANOVA with Tukey correction. e ChIP-qPCR analysis performed at indicated loci with anti-GR, anti-LSD1 or anti-H3K9me2 antibodies in skeletal muscle of wild-type mice at 0, 3, 6, 12 and 24 h of food deprivation. The promoter region of Pax7 and an unrelated region within the Ddit4 locus were used as negative controls. N = 3 mice. Mean ± SEM. One-way ANOVA with Tukey correction, with the following annotation on the figure. a: fed vs starved, p < 0.05. b: fed vs starved, p < 0.001. c: starved 3 h vs other time points, p < 0.05. d: starved 3 h vs other time points, p < 0.01. e: starved 3 h vs other time points, p < 0.001. Exact p values are detailed in “Source Data”. Source data are provided as a Source Data file.
