Fig. 8: LSD1 inhibition does not impair dexamethasone-induced improvement of experimental colitis symptoms.
From: LSD1 inhibition circumvents glucocorticoid-induced muscle wasting of male mice
a–c Water consumption (a), weight change (b) and clinical score (c) of 12-week-old DSS-exposed wild-type mice treated with vehicle (DSS+vehicle), DEX (DSS + DEX), DEX with CC-90011 (DSS + DEX + CC) or CC-90011 (DSS + CC). N = 5 mice per condition. Mean ± SEM. Two-way ANOVA with Tukey correction. d, e Colon representative images (d) and length measurement (e) of mice exposed to indicated treatments. N = 5 mice per condition. Basal values are represented by a dashed line. Mean ± SEM. One-way ANOVA, correction for multiple comparisons. f Relative transcript levels of IL-6 determined in colon. N = 5 mice per condition, all the individual values of the technical replicates are presented on the graph. Basal values are represented by a dashed line. Mean ± SEM. One-way ANOVA, correction for multiple comparisons. g Representative H&E staining of colons of mice from various groups. Red and black arrows denote inflammatory cells and exfoliated epithelial cells, respectively. GC goblet cell, LP lumina propria, SM muscularis mucosae composed of smooth muscle fibers. Scale bars, 100 µm. h Spleen weight of mice from various groups. N = 5 mice per condition. Basal values are represented by a dashed line. Mean ± SEM. Two-way ANOVA with Tukey correction. i Flow cytometry analysis of the lymphoid and the myeloid lineages from spleens of mice from various groups. N = 3 mice per condition. Basal values are represented by a dashed line. Mean ± SEM. Two-way ANOVA with Tukey correction. j Blood count analysis of mice from various groups. RBC: red blood cells. N = 5 mice per condition. Basal values are represented by a dashed line. Mean ± SEM. Two-way ANOVA with Tukey correction. k Gastrocnemius, tibialis, quadriceps and soleus mass relative to body weight (BW) of mice from various groups. N = 5 mice per condition. Basal values are represented by a dashed line. Mean ± SEM. Two-way ANOVA with Tukey correction. Source data are provided as a Source Data file.
