Fig. 3: Dynamic TIRF imaging reveals age-associated trafficking deficits of CaV1.2.
From: BIN1 knockdown rescues systolic dysfunction in aging male mouse hearts
a, b Representative TIRF images of transduced Cavβ2a-paGFP young (a) and old (b) ventricular myocytes before (top) and after ISO (bottom). Channel populations that were inserted (red), endocytosed (blue), or static (white) during the ISO treatment are represented to the right. c, d Dot-plots summarizing the quantification of inserted (c) and endocytosed (d) Cavβ2a-paGFP populations. e is a compilation of the data to show the relative % of the channel pool that is inserted, endocytosed, and static. f is a summary plot showing the net % change of plasma membrane Cavβ2a-paGFP after ISO for each cohort of young and old myocytes. N-numbers for data in (c–f ) are as follows: young (N = 3, n = 9) and old (N = 3, n = 10). Statistical analysis was performed on data in (c–f ) using unpaired two-tailed Student’s t-tests. Young data in (c–f ) are from JAX young. Data are presented as mean ± SEM. Source data are provided in the Source Data file.
