Fig. 10: Schema for hepatocyte dedifferentiation and the specific properties of dediHeps. | Nature Communications

Fig. 10: Schema for hepatocyte dedifferentiation and the specific properties of dediHeps.

From: Hepatocytes differentiate into intestinal epithelial cells through a hybrid epithelial/mesenchymal cell state in culture

Fig. 10

In monolayer culture, hepatocytes isolated from adult mouse livers dedifferentiate into dediHeps as a result of inactivation of the Hippo signaling pathway. These dediHeps exhibit a hybrid E/M phenotype and Vim-dependent propagation, express Cdx2 through activation of the BMP signaling pathway, and give rise to hepatocytes and intestinal epithelial cells under 3D culture conditions, which can respectively reconstitute hepatic tissues and colonic epithelial tissues after transplantation. Forced Cdx2 expression in dediHeps facilitates their conversion to ISC-like cells that form BOs and give rise to multilineage intestinal epithelial cells.

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