Fig. 2: Decrease of peripheral virus shedding in meganuclease-treated mice.
From: Gene editing for latent herpes simplex virus infection reduces viral load and shedding in vivo
Experimental timeline of ocular infection, meganuclease treatment and viral reactivations with JQ1. a Experiment 1 (n = 10 per group). HSV loads in SCGs (b: p = 0.0057) and TGs (c). Percent decrease of HSV loads in treated mice compared to control mice and statistical analysis (unpaired one-tailed Mann–Whitney test with ns: not significant, **p < 0.01) are indicated. Gene editing at the m5 target site of residual virus quantified by T7E1 assay in SCG and TG from dual meganuclease-treated mice (d). HSV titers in eye swabs collected daily from day 1 to 4 post JQ1 reactivation from control (e) and dual meganuclease-treated (f) infected mice. Panels 2i-k show data for both SCG and both TG from each mouse. Area under the curve (AUC) analysis (g) with p value (unpaired one-tailed Mann–Whitney test). AAV loads are shown in Supplemental Fig. 9c, d. Each graph shows individual and mean values with standard deviation. Source data are provided as a Source Data file.
