Fig. 2: Acinar-derived insulin-producing (ADIP) cells infiltrate pre-existing endocrine islets.

a–f Imaging of tamoxifen-induced ElaCreERT2,R26^Tom mice treated with FAKi showing Tom⁺/insulin⁺ cells within the endocrine islets. n = 5 mice from 3 independent experiments. Fluorescent imaging of Tomato in conjunction with insulin and amylase (a), insulin and GLUT2 (b), insulin, and PDX1 showing islet infiltrated with three Tom⁺ cells (c). Confocal fluorescent imaging of Tomato in conjunction with insulin and Nkx6.1 (d–f). d–f Higher magnification of three Tom⁺ cells shown in (Supplementary Fig. 2e). Arrows highlight a Tom⁺/Nkx6.1^-/Ins^- cell (d), a Tom⁺/Nkx6.1⁺/Ins⁺ cell (e) and a Tom⁺/Nkx6.1⁺/Ins^- cell (f). g Fluorescent imaging of Tomato in conjunction with BrdU on tissues obtained from tamoxifen-induced ElaCreERT2,R26^Tom mice treated with FAKi showing the absence of proliferation among Tom⁺ cells within the endocrine islets. Dotted line mark and islet. Arrowheads in (g) highlight Tom⁺ cells inside the islet. Arrows in (g) show BrdU⁺ acinar cells. isl: islet. Scale bar = 20 μm. n = 5 mice from 3 independent experiments.