Fig. 6: scRNA-seq analysis of FAKi-treated acinar- and β-cells.
From: Acinar to β-like cell conversion through inhibition of focal adhesion kinase
a UMAPs of the identified acinar or β-cell clusters defined by specific color showing vehicle- vs FAKi-treated. Arrow shows the formed bridge between acinar cluster 2 and β-cells in FAKi-treated samples. b Gene expression dot plots of selected marker genes across cluster 1 vs cluster 2 acinar cells in vehicle- versus FAKi-treated samples. c Fluorescent imaging for detection of amylase, insulin, and glucagon in wild type control pancreas. Arrowheads highlight two peri-islet acinar cells with low amylase expression. Scale bar = 20 μm, n = 5. d Single-cell gene expression of known pancreatic or acinar markers. e Heatmap showing the gene expression dynamics of the acinar and β-cell from acinar cells onward. Genes were grouped based on k-means clustering, and select genes are labeled on the right side of the plot.
