Fig. 1: Substrate specificities of P. fendleri DGATs. | Nature Communications

Fig. 1: Substrate specificities of P. fendleri DGATs.

From: Identification of triacylglycerol remodeling mechanism to synthesize unusual fatty acid containing oils

Fig. 1

A, B Acyl-CoA specificities of PfeDGAT1 and PfeDGAT2 with [14C]1,2-rac-diolein (20 nmol) and [14C]1HFA-rac-DAG (20 nmol) substrates. The [14C]1HFA-rac-DAG was derived from P. fenderli 2HFA-TAG and thus contains predominantly 20:1OH as the HFA. Acyl-CoA was added at a concentration of 5 nmol. C, D sn-1,2- and sn-2,3-DAG enantiomer specificities of PfeDGAT1 and PfeDGAT2 with [14C]18:1-CoA (1 nmol) and [14C]20:1-OH-CoA (5 nmol). Activities are assayed using microsomes preparation from yeast expressing the enzymes. All the results are shown as mean ± SD of three independent assay reactions. Source data underlying A–D are provided in the source data file.

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