Fig. 10: Organization of TAG biosynthesis through TAG remodeling in P. fendleri. | Nature Communications

Fig. 10: Organization of TAG biosynthesis through TAG remodeling in P. fendleri.

From: Identification of triacylglycerol remodeling mechanism to synthesize unusual fatty acid containing oils

Fig. 10

Dashed arrows are acyl transfers, blue are common fatty acids, and red is hydroxy fatty acids. Solid lines involve glycerol backbone flux. Enzymes characterized by activity, interaction, and localization in this study are in blue boxes. The model utilizes at least two PC pools, one involved in acyl editing and fatty acid hydroxylation/desaturation. The second pool of PC is involved in PC-derived DAG biosynthesis containing common fatty acids. The model also involves at least three pools of DAG: (1) de novo sn-1,2-DAG produced by the Kennedy pathway utilized for PC synthesis; (2) PC-derived sn-1,2-DAG utilized by DGAT1 to produce 1HFA-TAG containing the HFA at the sn-3 position; (3) TAGLL1 activity on 1HFA-TAG produces a sn-2,3-DAG with the HFA at the sn-3 position, that is utilized by DGAT2 to produce the final 2HFA-TAG. Abbreviations not in main text: F.A.S., fatty acid synthesis; G3P; glycerol-3-phosphate.

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